We obtain the maximal regularity for the mixed Dirichlet-conormal problem in cylindrical domains with time-dependent separations, which is the first of its kind. The boundary of the domain is assumed to be Reifenberg-flat and the separation is locally sufficiently close to a Lipschitz function of $m$ variables, where $m=0,ldots,d-2$, with respect to the Hausdorff distance. We consider solutions in both $L_p$-based Sobolev spaces and $L_{q,p}$-based mixed-norm Sobolev spaces.