This paper studies the gamma-ray spectra of positron annihilation processes in a series of molecules. The results show that the average valence electron energy of the molecules has a linear correlation with the full width at half maximum (FWHM) of the gamma-ray spectra. In addition, we defined a new physical quantity Average Doppler Shift (ADS), which can be used as the eigenvalue to describe the characteristics of the gamma-ray spectra. Since ADS contains all the information about the gamma-ray spectra, it can more accurately represent the characteristics of the gamma-ray spectra. For a series of molecules, this paper compares the ADS and FWHM of their gamma-ray spectra and the average valence electron energy. The results show that ADS has a linear correlation with the average valence electron energy and the FWHM. Further, this proves that the annihilation mainly occurs on valence electrons, and it also illustrates that the ADS has certain applicability. It is expected that this will provide us with a deeper understanding of the positron annihilation process.