We report detections of atomic species in the atmosphere of MASCARA-2 b, using the first transit observations obtained with the newly commissioned EXPRES spectrograph. EXPRES is a highly stabilised optical echelle spectrograph, designed to detect stellar reflex motions with amplitudes down to 30 cm/s, and was recently deployed at the Lowell Discovery Telescope. By analysing the transmission spectrum of the ultra-hot Jupiter MASCARA-2 b using the cross-correlation method, we confirm previous detections of Fe I, Fe II and Na I, which likely originate in the upper regions of the inflated atmosphere. In addition, we report significant detections of Mg I and Cr II. The absorption strengths change slightly with time, possibly indicating different temperatures and chemistry in the day-side and night-side terminators. Using the effective stellar line-shape variation induced by the transiting planet, we constrain the projected spin-orbit misalignment of the system to $1.6pm3.1$ degrees, consistent with an aligned orbit. We demonstrate that EXPRES joins a suite of instruments capable of phase-resolved spectroscopy of exoplanet atmospheres.