Many-body localization (MBL) describes a quantum phase where an isolated interacting system subject to sufficient disorder displays non-ergodic behavior, evading thermal equilibrium that occurs under its own dynamics. Previously, the thermalization-MBL transition has been largely characterized with the growth of disorder. Here, we explore a new axis, reporting on an energy resolved MBL transition using a 19-qubit programmable superconducting processor, which enables precise control and flexibility of both disorder strength and initial state preparations. We observe that the onset of localization occurs at different disorder strengths, with distinguishable energy scales, by measuring time-evolved observables and many-body wavefunctions related quantities. Our results open avenues for the experimental exploration of many-body mobility edges in MBL systems, whose existence is widely debated due to system size finiteness, and where exact simulations in classical computers become unfeasible.