Clusterix 2.0 is a web-based, Virtual Observatory-compliant, interactive tool for the determination of membership probabilities in stellar clusters based on proper motion data using a fully non-parametric method. In the area occupied by the cluster, the frequency function is made up of two contributions: cluster and field stars. The tool performs an empirical determination of the frequency functions from the Vector-Point Diagram without relying in any previous assumption about their profiles. Clusterix 2.0 allows to search in an interactive way the appropriate spatial areas until an optimal separation of the two populations is obtained. Several parameters can be adjusted to make the calculation computationally feasible without interfering in the quality of the results. The system offers the possibility to query different catalogues, such as Gaia, or upload the user own data. The results of the membership determination can be sent via SAMP to VO tools like TopCat. We apply Clusterix 2.0 to several open clusters with different properties and environments to show the capabilities of the tool: an area of five degrees around NGC 2682 (M 67), an old, well known cluster; a young cluster NGC 2516 with a striking elongate structure extended up to four degrees; NGC 1750 & NGC 1758, a pair of partly overlapping clusters; in the area of NGC 1817 we confirm a little-known cluster, Juchert 23; and in an area with many clusters we disentangle the existence of two overlapping clusters where only one was previously known: Ruprecht 26 and the new, Clusterix 1.