We present the relation between the sphaleron energy and the gravitational wave signals from a first order electroweak phase transition. The crucial ingredient is the scaling law between the sphaleron energy at the temperature of the phase transition and that at zero temperature. We estimate the baryon number preservation criterion, and observe that for a sufficiently strong phase transition, it is possible to probe the electroweak sphaleron using measurements of future space-based gravitational wave detectors.
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