In the first part of this paper, we consider the problem of fill-in of nonnegative scalar curvature (NNSC) metrics for a triple of Bartnik data $(Sigma,gamma,H)$. We prove that given a metric $gamma$ on $mathbf{S}^{n-1}$ ($3leq nleq 7$), $(mathbf{S}^{n-1},gamma,H)$ admits no fill-in of NNSC metrics provided the prescribed mean curvature $H$ is large enough (Theorem ref{Thm: no fillin nonnegative scalar 2}). Moreover, we prove that if $gamma$ is a positive scalar curvature (PSC) metric isotopic to the standard metric on $mathbf{S}^{n-1}$, then the much weaker condition that the total mean curvature $int_{mathbf S^{n-1}}H,mathrm dmu_gamma$ is large enough rules out NNSC fill-ins, giving an partially affirmative answer to a conjecture by Gromov (see P.,23 in cite{Gromov4}). In the second part of this paper, we investigate the $theta$-invariant of Bartnik data and obtain some sufficient conditions for the existence of PSC fill-ins.