We study the two-point correlation functions of chiral/anti-chiral operators in $N=2$ supersymmetric Yang-Mills theories on $R^4$ with gauge group SU(N) and $N_f$ massless hypermultiplets in the fundamental representation. We compute them in perturbation theory, using dimensional regularization up to two loops, and show that field-theory observables built out of dimensionless ratios of two-point renormalized correlators on $R^4$ are in perfect agreement with the same quantities computed using localization on the four-sphere, even in the non-conformal case $N_f ot=2N$.