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High-resolution long-working-distance reference-free holographic microscopy exploiting speckle-correlation scattering matrix

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 Added by YongKeun Park
 Publication date 2018
  fields Physics
and research's language is English




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Using conventional refraction-based optical lens, it is challenging to achieve both high-resolution imaging and long-working-distance condition. To increase the numerical aperture of a lens, the working distance should be compensated, and vice versa. Here we propose and demonstrate a new concept in optical microscopy that can achieve both high-resolution imaging and long-working-distance conditions by utilising a scattering layer instead of refractive optics. When light passes through a scattering layer, it creates a unique interference pattern. To retrieve the complex amplitude image from the interference pattern without introducing a reference beam, we utilised a speckle-correlation scattering matrix method. This property enables holographic microscopy without any lens or external reference beam. Importantly, the proposed method allows high-resolution imaging with a long working distance beyond what a conventional objective lens can achieve. As an experimental verification, we imaged various microscopic samples and compared their performance with off-axis digital holographic microscopy.



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