No Arabic abstract
Using conventional refraction-based optical lens, it is challenging to achieve both high-resolution imaging and long-working-distance condition. To increase the numerical aperture of a lens, the working distance should be compensated, and vice versa. Here we propose and demonstrate a new concept in optical microscopy that can achieve both high-resolution imaging and long-working-distance conditions by utilising a scattering layer instead of refractive optics. When light passes through a scattering layer, it creates a unique interference pattern. To retrieve the complex amplitude image from the interference pattern without introducing a reference beam, we utilised a speckle-correlation scattering matrix method. This property enables holographic microscopy without any lens or external reference beam. Importantly, the proposed method allows high-resolution imaging with a long working distance beyond what a conventional objective lens can achieve. As an experimental verification, we imaged various microscopic samples and compared their performance with off-axis digital holographic microscopy.
We propose a three-dimensional holographic reconstruction procedure applicable with no a priori knowledge about the recording conditions enabling distortion-free three-dimensional object reconstruction.
We present a pair of optimized objective lenses with long working distances of 117~mm and 65~mm respectively that offer diffraction limited performance for both Cs and Rb wavelengths when imaging through standard vacuum windows. The designs utilise standard catalog lens elements to provide a simple and cost-effective solution. Objective 1 provides $mathrm{NA}=0.175$ offering 3~$mu$m resolution whilst objective 2 is optimized for high collection efficiency with $mathrm{NA}=0.29$ and 1.8~$mu$m resolution. This flexible design can be further extended for use at shorter wavelengths by simply re-optimising the lens separations.
Rapidly and randomly drifted reference frames will shorten the link distance and decrease the secure key rate of realistic quantum key distribution (QKD) systems. However, an actively or inappropriately implemented calibration scheme will increase complexity of the systems and may open security loopholes. In this article, we present a free-running reference-frame-independent (RFI) QKD scheme, where measurement events are classified into multiple slices with the same misalignment variation of reference frames and each slice performs the post-processing procedure individually. We perform the free-running RFI QKD experiment with a fiber link of 100km and the misalignment of the reference frame between Alice and Bob is varied more than 29 periods in a 50.7-hour experiment test. The average secure key rate is about 734 bps with a total loss of 31.5 dB, which achieves the state-of-art performance of the long-distance RFI QKD implementations. Our free-running RFI scheme can be efficiently adapted into the satellite-to-ground and drone based mobile communication scenarios, as it can be performed with rapidly varying reference frame and a loss more than 40 dB, where no secure key can be obtained by the original RFI scheme.
Chip-based Evanescent Light Scattering (cELS) utilizes the multiple modes of a high-index contrast optical waveguide to provide a near-field illumination for unlabeled samples. The scattered light off the sample is engineered to have random phase differences within the integration time of the camera to mitigate the coherent speckle noise, thus enabling label-free superior-contrast imaging of weakly scattering nanosized specimens such as extra-cellular vesicles (EVs) and liposomes, dynamics of living HeLa cells etc. The article explains and validates experimentally the physics behind cELS, by demonstrating a wide highly multi-moded straight waveguide as a partially coherent light source. Next, to circumvent the diffraction-limit in cELS, intensity-fluctuation based algorithms are employed with spatially incoherent light engineered via multiple-arms waveguide chip. The proof-of-concept results are demonstrated on 100 nm polystyrene beads. We believe cELS will further propel the nascent field of label-free super-resolution microscopy finding applications in cell biology.
Quantitative phase microscopy (QPM) has found significant applications in the field of biomedical imaging which works on the principle of interferometry. The theory behind achieving interference in QPM with conventional light sources such as white light and lasers is very well developed. Recently, the use of dynamic speckle illumination (DSI) in QPM has attracted attention due to its advantages over conventional light sources such as high spatial phase sensitivity, single shot, scalable field of view (FOV) and resolution. However, the understanding behind obtaining interference fringes in QPM with DSI has not been convincingly covered previously. This imposes a constraint on obtaining interference fringes in QPM using DSI and limits its widespread penetration in the field of biomedical imaging. The present article provides the basic understanding of DSI through both simulation and experiments that is essential to build interference optical microscopy systems such as QPM, digital holographic microscopy and optical coherence tomography. Using the developed theory of DSI we demonstrate its capabilities of using non-identical objective lenses in both arms of the interference microscopy without degrading the interference fringe contrast and providing the flexibility to use user-defined microscope objective lens. It is also demonstrated that the interference fringes are not washed out over a large range of optical path difference (OPD) between the object and the reference arm providing competitive edge over low temporal coherence light sources. The theory and explanation developed here would enable wider penetration of DSI based QPM for applications in biology and material sciences.