If one removes any emph{ad hoc} symmetry assumptions, the general two Higgs doublet model should have additional Yukawa interactions independent from fermion mass generation, in general involving flavor changing neutral Higgs couplings. These extra couplings can affect the discovered Higgs boson $h$ through fermion loop contributions. We calculate the renormalized $hZZ$ coupling at one-loop level %by on-shell and minimal subtraction scheme, and evaluate the dependence on heavy Higgs boson mass and extra Yukawa coupling $rho_{tt}$. Precision measurements at future colliders can explore the parameter space, and can give stronger bound on $rho_{tt}$ than the current bound from flavor experiments. As a side result, we find that if $rho_{tt}cosgamma < 0$, where $cosgamma$ is the exotic Higgs component of $h$, the $rho_{tt}$-induced top loop contribution cancels against bosonic loop contributions, and one may have alignment without decoupling, namely $sin(-gamma) simeq 1$, but exotic scalar bosons could have masses at several hundred GeV.
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