In this paper we analyze the effect of dynamical three-dimensional MHD turbulence on test particle acceleration, and compare how this evolving system affects particle energization by current sheets interaction, against frozen-in-time fields. To do this we analize the ensamble particle acceleration for static electromagnetic fields extracted from direct numerical simulations of the MHD equations, and compare with the dynamical fields. We show that a reduction in particle acceleration in the dynamical model results from the particle trapping in the field lines, which forces the particles to remain in a moving current sheet that suppress the longer exposure at the strong electric field gradients located between structures, which is an efficient particle acceleration mechanism. In addition, we analize the effect of anisotropy caused by the mean magnetic field. It is well known that for sufficiently strong external fields, the system suffers a transition towards a two-dimensional flow. This causes an increment in the size of the coherent structures, resulting in a magnetized state of the particles and the reduction of the particle energization.