The recently-developed notion of parity-time (PT) symmetry in optical systems with a controlled gain-loss interplay has spawned an intriguing way of achieving optical behaviors that are presently unattainable with standard arrangements. In most experimental studies so far, however, the implementations rely highly on the advances of nanotechnologies and sophisticated fabrication techniques to synthesize solid-state materials. Here, we report the first experimental demonstration of optical anti-PT symmetry, a counterpart of conventional PT symmetry, in a warm atomic-vapor cell. By exploiting rapid coherence transport via flying atoms, our scheme illustrates essential features of anti-PT symmetry with an unprecedented precision on phase-transition threshold, and substantially reduces experimental complexity and cost. This result represents a significant advance in non-Hermitian optics by bridging a firm connection with the field of atomic, molecular and optical physics, where novel phenomena and applications in quantum and nonlinear optics aided by (anti-)PT symmetry could be anticipated.