We describe a method for sensing short range forces using matter wave interference in dielectric nanospheres. When compared with atom interferometers, the larger mass of the nanosphere results in reduced wave packet expansion, enabling investigations of forces nearer to surfaces in a free-fall interferometer. By laser cooling a nanosphere to the ground state of an optical potential and releasing it by turning off the optical trap, acceleration sensing at the $10^{-8}$m/s$^2$ level is possible. The approach can yield improved sensitivity to Yukawa-type deviations from Newtonian gravity at the $5$ $mu$m length scale by a factor of $10^4$ over current limits.
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