In this article, we establish sufficient conditions for the regularity of solutions of Navier-Stokes equations based on one of the nine entries of the gradient tensor. We improve the recently results of C.S. Cao, E.S. Titi (Arch. Rational Mech.Anal. 202 (2011) 919-932) and Y. Zhou, M. Pokorn$acute{y}$ (Nonlinearity 23, 1097-1107 (2010)).
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