We present time-resolved spectroscopy of the eclipsing, short period cataclysmic variable CTCV J1300-3052. Using absorption features from the secondary star, we determine the radial velocity semi-amplitude of the secondary star to be K2 = 378 pm 6 km/s, and its projected rotational velocity to be v sin i = 125 pm 7 km/s. Using these parameters and Monte Carlo techniques, we obtain masses of M1 = 0.79 pm 0.05 MSun for the white dwarf primary and M2 = 0.198 pm 0.029 MSun for the M-type secondary star. These parameters are found to be in excellent agreement with previous mass determinations found via photometric fitting techniques, supporting the accuracy and validity of photometric mass determinations in short period CVs.