We present new investigation of the Lamb shift (2P_{1/2}-2S_{1/2}) in muonic deuterium (mu d) atom using the three-dimensional quasipotential method in quantum electrodynamics. The vacuum polarization, nuclear structure and recoil effects are calculated with the account of contributions of orders alpha^3, alpha^4, alpha^5 and alpha^6. The results are compared with earlier performed calculations. The obtained numerical value of the Lamb shift 202.4139 meV can be considered as a reliable estimate for the comparison with forthcoming experimental data.