We report the observation of many-body interaction effects for a homonuclear bosonic mixture in a three-dimensional optical lattice with variable state dependence along one axis. Near the superfluid-to-Mott insulator transition for one component, we find that the presence of a second component can reduce the apparent superfluid coherence, most significantly when it either experiences a strongly localizing lattice potential or none at all. We examine this effect by varying the relative populations and lattice depths, and discuss the observed behavior in view of recent proposals for scattering from impurities and of atom-phonon coupling for atoms immersed in a superfluid.
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