A rich harvest of RR Lyrae stars has been identified for the first time in B514, a metal-poor ([Fe/H] = 1.95 +/- 0.10 dex) globular cluster of the Andromeda galaxy (M31), based on Hubble Space Telescope Wide Field Planetary Camera 2 and Advanced Camera for Surveys time-series observations. We have detected and derived periods for 89 RR Lyrae stars (82 fundamental-mode -RRab- and 7 first-overtone -RRc- pulsators, respectively) among 161 candidate variables identified in the cluster. The average period of the RR Lyrae variables (<Pab> = 0.58 days and <Pc> = 0.35 days, for RRab and RRc pulsators, respectively) and the position in the period-amplitude diagram both suggest that B514 is likely an Oosterhoff type I cluster. This appears to be in disagreement with the general behaviour of the metal-poor globular clusters in the Milky Way, which show instead Oosterhoff type II pulsation properties. The average apparent magnitude of the RR Lyrae stars sets the mean level of the cluster horizontal branch at <V(RR)> = 25.18 +/- 0.02 (sigma=0.16 mag, on 81 stars). By adopting a reddening E(B-V) = 0.07 +/- 0.02 mag, the above metallicity and M_V=0.44 +/- 0.05 mag for the RR Lyrae variables of this metallicity, we derive a distance modulus of mu_0=24.52 +/- 0.08 mag, corresponding to a distance of about 800 +/- 30 kpc, based on a value of M_V that sets mu_0(LMC)=18.52.
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