We propose and demonstrate a novel method to generate a large-amplitude coherent-state superposition (CSS) via ancilla-assisted photon-subtraction. The ancillary mode induces quantum interference of indistinguishable processes, widening the controllability of quantum superposition at the conditional output. We demonstrate the concept in the time domain, by a simple time-separated two-photon subtraction from cw squeezed light. We observe the largest CSS ever reported without any corrections, which will enable various quantum information applications with CSS states.