We calculate the mean and variance of net-baryon number and net-electric charge distributions from Quantum Chromodynamics (QCD) using a next-to-leading order Taylor expansion in terms of temperature and chemical potentials. We compare these expansions with experimental data from STAR and PHENIX, determine the freeze-out temperature in the limit of vanishing baryon chemical potential, and, for the first time, constrain the curvature of the freeze-out line through a direct comparison between experimental data on net-charge fluctuations and a QCD calculation. We obtain a bound on the curvature coefficient, kappa_2^f < 0.011, that is compatible with lattice QCD results on the curvature of the QCD transition line.
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