We report results from the MuLan measurement of the positive muon lifetime. The experiment was conducted at the Paul Scherrer Institute using a time-structured surface muon beam and a segmented plastic scintillator array. Two different in-vacuum muon stopping targets were used: a ferromagnetic foil with a large internal magnetic field and a quartz crystal in a moderate external magnetic field. From a total of 1.6 x 10^{12} decays, we obtained the muon lifetime tau_mu = 2196980.3(2.2) ps (1.0 ppm) and Fermi constant G_F = 1.1663787(6) x 10^{-5} GeV^{-2} (0.5 ppm).