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تسجيل مستخدم جديدDiffusible crosslinkers cause superexponential friction forces
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The mitotic spindle lies at the heart of the spatio-temporal control over cellular components during cell division. The spindle consists of microtubules, which are not only crosslinked by motor proteins but also by passive binding proteins. These passive crosslinkers stabilize the highly dynamic mitotic spindle by generating friction forces between sliding filaments. However, it remains unclear how the friction coefficient depends on the number of crosslinkers and the size of the overlap between the microtubules. Here, we use theory and computer simulations to study the friction between two filaments that are crosslinked by passive proteins, which can hop between neighboring binding sites while physically excluding each other. The simulations reveal that the movement of one microtubule relative to the other is limited by free-energy barrier crossings, causing rare and discrete jumps of the microtubule that span the distance between adjacent crosslinker binding sites. We derive an exact analytical expression for the free-energy landscape and identify the reaction coordinate that governs the relative movement, which allows us to determine the effective barrier height for the microtubule jumps. Both through simulations and reaction rate theory, we make the experimentally testable prediction that the friction between the microtubules increases superexponentially with the density of crosslinkers.
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