In this paper, we study biharmonic hypersurfaces in a product of an Einstein space and a real line. We prove that a biharmonic hypersurface with constant mean curvature in such a product is either minimal or a vertical cylinder generalizing a result of cite{OW} and cite{FOR}. We derived the biharmonic equation for hypersurfaces in $S^mtimes mathbb{R}$ and $H^mtimes mathbb{R}$ in terms of the angle function of the hypersurface, and use it to obtain some classifications of biharmonic hypersurfaces in such spaces. These include classifications of biharmonic hypersurfaces which are totally umbilical or semi-parallel for $mge 3$, and some classifications of biharmonic surfaces in $S^2times mathbb{R}$ and $H^2times mathbb{R}$ which are constant angle or belong to certain classes of rotation surfaces.