We have measured the quantum depletion of an interacting homogeneous Bose-Einstein condensate, and confirmed the 70-year old theory of N.N. Bogoliubov. The observed condensate depletion is reversibly tuneable by changing the strength of the interparticle interactions. Our atomic homogeneous condensate is produced in an optical-box trap, the interactions are tuned via a magnetic Feshbach resonance, and the condensed fraction probed by coherent two-photon Bragg scattering.