The Suzaku X-ray satellite observed the nearby spiral galaxy NGC 4258 for a total good exposure time of 100 ks. We present an analysis of the Suzaku XIS data, in which we confirm that the 0.5--2 keV spectra of the interstellar medium (ISM) are well-represented by a two-temperature model. The cool and hot ISM temperatures are 0.23+-0.02 and 0.59 +-0.01 keV, respectively. Suzakus excellent spectral sensitivity enables us to measure the metal abundances of O, Ne, Mg, Si and Fe of the ISM for the first time. The resultant abundance pattern of O, Mg, Si, and Fe is consistent with that of the new solar abundance table of Lodders (2003), rather than Anders & Grevesse (1989). This suggests that the metal enrichment processes of NGC 4258 and of our Galaxy are similar.