The decay K- -> pi0 e- nu has been studied using in-flight decays detected with ISTRA+ setup operating at the 25 GeV negative secondary beam of the U-70 PS. About 130K events were used for the analysis. The lambda_+ parameter of the vector formfactor has been measured: lambda_+ = 0.0293 +- 0.0015(stat) +- 0.002(syst). The limits on the possible tensor and scalar couplings have been derived: f_T/f_+(0)=-0.044 +0.059 -0.057 (stat) f_S/f_+(0)=-0.020 +0.025 -0.016 (stat)