We propose a method for the dynamical control in three-level open systems and realize it in the experiment with a superconducting qutrit. Our work demonstrates that in the Markovian environment for a relatively long time (3 us), the systemic populations or coherence can still strictly follow the preset evolution paths. This is the first experiment for precisely controlling the Markovian dynamics of three-level open systems, providing a solid foundation for the future realization of dynamical control in multiple open systems. An instant application of the techniques demonstrated in this experiment is to stabilize the energy of quantum batteries.