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تسجيل مستخدم جديدEffect of Serum Starvation on Rheology of Cell Monolayers
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The rheological properties of cells and tissues are central to embryonic development and homoeostasis in adult tissues and organs and are closely related to their physiological activities. In this work, we present our study of rheological experiments on cell monolayer under serum starvation compared to that of healthy cell monolayer with full serum. The normal functioning of cells depends on the micronutrient supply provided by the serum in the growth media. Serum starvation is one of the most widely used procedures in cell biology. Serum deficiency may lead to genomic instability, variation in protein expression, chronic diseases, and some specific types of cancers. However, the effect of deprivation of serum concentration on the material properties of cells is still unknown. Therefore, we performed the macro-rheology experiments to investigate the effect of serum starvation on a fully confluent Madin Darby Canine Kidney (MDCK) cell monolayer. The material properties such as storage modulus (G) and loss modulus (G), of the monolayer, were measured using oscillatory shear experiments under serum-free (0% FBS) and full serum (10% FBS) conditions. Additionally, the step strain experiments were performed to gain more insights into the viscoelastic properties of the cell monolayer. Our results indicate that without serum, the loss and storage moduli decrease and do not recover fully even after small deformation. This is because of the lack of nutrients, which may result in many permanent physiological changes. Whereas, the healthy cell monolayer under full serum condition, remains strong & flexible, and can fully recover even from a large deformation at higher strain.
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