We present eight new light curves of the transiting extra-solar planet HAT-P-25b obtained from 2013 to 2016 with three telescopes at two observatories. We use the new light curves, along with recent literature material, to estimate the physical and orbital parameters of the transiting planet. Specifically, we determine the mid-transit times (T$_{C}$) and update the linear ephemeris, T$_{C[0]}$=2456418.80996$pm$0.00025 [$mathrm{BJD}_mathrm{TDB}$] and P=3.65281572$pm$0.00000095 days. We carry out a search for transit timing variations (TTVs), and find no significant TTV signal at the $Delta T=$80 s-level, placing a limit on the possible strength of planet-planet interactions ($mathrm{TTV_{G}}$). In the course of our analysis, we calculate the upper mass-limits of the potential nearby perturbers. Near the 1:2, 2:1, and 3:1 resonances with HAT-P-25b, perturbers with masses greater than 0.5, 0.3, and 0.5 $mathrm{M_{oplus}}$ respectively, can be excluded. Furthermore, based on the analysis of TTVs caused by light travel time effect (LTTE) we also eliminate the possibility that a long-period perturber exists with $M_{rm p}> 3000 ,mathrm{M_{J}}$ within $a=11.2,{rm AU}$ of the parent star.
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