Experimental and numerical study of the steady-state cyclonic vortex from isolated heat source in a rotating fluid layer is described. The structure of laboratory cyclonic vortex is similar to the typical structure of tropical cyclones from observational data and numerical modelling including secondary flows in the boundary layer. Differential characteristics of the flow were studied by numerical simulation using CFD software FlowVision. Helicity distribution in rotating fluid layer with localized heat source was analysed. Two mechanisms which play role in helicity generation are found. The first one is the strong correlation of cyclonic vortex and intensive upward motion in the central part of the vessel. The second one is due to large gradients of velocity on the periphery. The integral helicity in the considered case is substantial and its relative level is high.