In this paper, we report the detections of stellar variabilities from the first 2-year observations of sky area of about 1300 square degrees from the Tsinghua University-NAOC Transient Survey (TNTS). A total of 1237 variable stars (including 299 new ones) were detected with brightness < 18.0 mag and magnitude variation >= 0.1 mag on a timescale from a few hours to few hundred days. Among such detections, we tentatively identified 661 RR Lyrae stars, 431 binaries, 72 Semiregular pulsators, 29 Mira stars, 11 slow irregular variables, 11 RS Canum Venaticorum stars, 7 Gamma Doradus stars, 5 long period variables, 3 W Virginis stars, 3 Delta Scuti stars, 2 Anomalous Cepheids, 1 Cepheid, and 1 nove-like star based on their time-series variability index Js and their phased diagrams. Moreover, we found that 14 RR Lyrae stars show the Blazhko effect and 67 contact eclipsing binaries exhibit the OConnell effect. Since the period and amplitude of light variations of RR Lyrae variables depend on their chemical compositions, their photometric observations can be used to investigate distribution of metallicity along the direction perpendicular to the Galactic disk. We find that the metallicity of RR Lyrae stars shows large scatter at regions closer to the Galactic plane (e.g., -3.0 < [Fe/H] < 0) but tends to converge at [Fe/H]~ -1.7 at larger Galactic latitudes. This variation may be related to that the RRAB Lyrae stars in the Galactic halo come from globular clusters with different metallicity and vertical distances, i.e. OoI and OoII populations, favoring for the dual-halo model.