اشترك بالحزمة الذهبية واحصل على وصول غير محدود شمرا أكاديميا
تسجيل مستخدم جديدWhole Exome Sequencing to Estimate Alloreactivity Potential Between Donors and Recipients in Stem Cell Transplantation
107
0
0.0
(
0
)
اسأل ChatGPT حول البحث
ﻻ يوجد ملخص باللغة العربية
Whole exome sequencing was performed on HLA-matched stem cell donors and transplant recipients to measure sequence variation contributing to minor histocompatibility antigen differences between the two. A large number of nonsynonymous single nucleotide polymorphisms were identified in each of the nine unique donor-recipient pairs tested. This variation was greater in magnitude in unrelated donors as compared with matched related donors. Knowledge of the magnitude of exome variation between stem cell transplant recipients and donors may allow more accurate titration of immunosuppressive therapy following stem cell transplantation.
قيم البحث
اقرأ أيضاً
With the development of high throughput sequencing technology, it becomes possible to directly analyze mutation distribution in a genome-wide fashion, dissociating mutation rate measurements from the traditional underlying assumptions. Here, we seque
nced several genomes of Escherichia coli from colonies obtained after chemical mutagenesis and observed a strikingly nonrandom distribution of the induced mutations. These include long stretches of exclusively G to A or C to T transitions along the genome and orders of magnitude intra- and inter-genomic differences in mutation density. Whereas most of these observations can be explained by the known features of enzymatic processes, the others could reflect stochasticity in the molecular processes at the single-cell level. Our results demonstrate how analysis of the molecular records left in the genomes of the descendants of an individual mutagenized cell allows for genome-scale observations of fixation and segregation of mutations, as well as recombination events, in the single genome of their progenitor.
The existence of doublets is a key confounder in single-cell RNA sequencing (scRNA-seq) data analysis. Computational methods have been developed for detecting doublets from scRNA-seq data. We developed an R package DoubletCollection to integrate the
installation and execution of eight doublet-detection methods. DoubletCollection also provides a unified interface to perform and visualize downstream analysis after doublet detection. Here, we present a protocol of using DoubletCollection to benchmark doublet-detection methods. This protocol can automatically accommodate new doublet-detection methods in the fast-growing scRNA-seq field.
According to the National Cancer Institute, there were 9.5 million cancer-related deaths in 2018. A challenge in improving treatment is resistance in genetically unstable cells. The purpose of this study is to evaluate unsupervised machine learning o
n classifying treatment-resistant phenotypes in heterogeneous tumors through analysis of single cell RNA sequencing(scRNAseq) data with a pipeline and evaluation metrics. scRNAseq quantifies mRNA in cells and characterizes cell phenotypes. One scRNAseq dataset was analyzed (tumor/non-tumor cells of different molecular subtypes and patient identifications). The pipeline consisted of data filtering, dimensionality reduction with Principal Component Analysis, projection with Uniform Manifold Approximation and Projection, clustering with nine approaches (Ward, BIRCH, Gaussian Mixture Model, DBSCAN, Spectral, Affinity Propagation, Agglomerative Clustering, Mean Shift, and K-Means), and evaluation. Seven models divided tumor versus non-tumor cells and molecular subtype while six models classified different patient identification (13 of which were presented in the dataset); K-Means, Ward, and BIRCH often ranked highest with ~80% accuracy on the tumor versus non-tumor task and ~60% for molecular subtype and patient ID. An optimized classification pipeline using K-Means, Ward, and BIRCH models was evaluated to be most effective for further analysis. In clinical research where there is currently no standard protocol for scRNAseq analysis, clusters generated from this pipeline can be used to understand cancer cell behavior and malignant growth, directly affecting the success of treatment.
A quantum model on the chemically and physically induced pluripotency in stem cells is proposed. Based on the conformational Hamiltonian and the idea of slow variables (molecular torsions) slaving fast ones the conversion from the differentiate state
to pluripotent state is defined as the quantum transition between conformational states. The transitional rate is calculated and an analytical form for the rate formulas is deduced. Then the dependence of the rate on the number of torsion angles of the gene and the magnitude of the rate can be estimated by comparison with protein folding. The reaction equations of the conformational change of the pluripotency genes in chemical reprogramming are given. The characteristic time of the chemical reprogramming is calculated and the result is consistent with experiments. The dependence of the transition rate on physical factors such as temperature, PH value and the volume and shape of the coherent domain is analyzed from the rate equation. It is suggested that by decreasing the coherence degree of some pluripotency genes a more effective approach to the physically induced pluripotency can be made.
Despite substantial potential to transform bioscience, medicine, and bioengineering, whole-cell models remain elusive. One of the biggest challenges to whole-cell models is assembling the large and diverse array of data needed to model an entire cell
. Thanks to rapid advances in experimentation, much of the necessary data is becoming available. Furthermore, investigators are increasingly sharing their data due to increased emphasis on reproducibility. However, the scattered organization of this data continues to hamper modeling. Toward more predictive models, we highlight the challenges to assembling the data needed for whole-cell modeling and outline how we can overcome these challenges by working together to build a central data warehouse.
سجل دخول لتتمكن من نشر تعليقات
التعليقات
جاري جلب التعليقات
سجل دخول لتتمكن من متابعة معايير البحث التي قمت باختيارها
