The quantum statistics of atoms is typically observed in the behavior of an ensemble via macroscopic observables. However, quantum statistics modifies the behavior of even two particles, inducing remarkable consequences that are at the heart of quantum science. Here we demonstrate near-complete control over all the internal and external degrees of freedom of two laser-cooled 87Rb atoms trapped in two optical tweezers. This full controllability allows us to implement a massive-particle analog of a Hong-Ou-Mandel interferometer where atom tunneling plays the role of a photon beamsplitter. We use the interferometer to probe the effect of quantum statistics on the two-atom dynamics under tunable initial conditions, chosen to adjust the degree of atomic indistinguishability. Our work thereby establishes laser-cooled atoms in optical tweezers as a new route to bottom-up engineering of scalable, low-entropy quantum systems.
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