With the uniquely high contrast within 0.1 (Delta mag(L) = 5-6.5 magnitudes) available using Sparse Aperture Masking (SAM) with NACO at VLT, we detected asymmetry in the flux from the Herbig Fe star HD 142527 with a barycenter emission situated at a projected separation of 88+/-5 mas (12.8+/-1.5 AU at 145 pc) and flux ratios in H, K, and L of 0.016+/-0.007, 0.012+/-0.008, 0.0086+/-0.0011 respectively (3-sigma errors) relative to the primary star and disk. After extensive closure-phase modeling, we interpret this detection as a close-in, low-mass stellar companion with an estimated mass of ~0.1-0.4 M_Sun. HD 142527 has a complex disk structure, with an inner gap imaged in both the near and mid-IR as well as a spiral feature in the outer disk in the near-IR. This newly detected low-mass stellar companion may provide a critical explanation of the observed disk structure.