We report simultaneous multi-frequency observations of the blazar PG 1553+113, that were carried out in March-April 2008. Optical, X-ray, high-energy (HE; greater than 100 MeV) gamma-ray, and very-high- energy (VHE; greater than 100 GeV) gamma-ray data were obtained with the KVA, REM, RossiXTE/ASM, AGILE and MAGIC telescopes. This is the first simultaneous broad-band (i.e., HE+VHE) gamma-ray observation of a blazar. The source spectral energy distribution derived combining these data shows the usual double-peak shape, and is interpreted in the framework of a synchrotron-self-Compton model.