We use high-quality VLA images of the Fanaroff & Riley Class I radio galaxy 3C 31 at six frequencies in the range 1365 to 8440MHz to explore the spatial scale and origin of the rotation measure (RM) fluctuations on the line of sight to the radio source. We analyse the distribution of the degree of polarization to show that the large depolarization asymmetry between the North and South sides of the source seen in earlier work largely disappears as the resolution is increased. We show that the depolarization seen at low resolution results primarily from unresolved gradients in a Faraday screen in front of the synchrotron-emitting plasma. We establish that the residual degree of polarization in the short-wavelength limit should follow a Burn law and we fit such a law to our data to estimate the residual depolarization at high resolution. We show that the observed RM variations over selected areas of 3C 31 are consistent with a power spectrum of magnetic fluctuations in front of 3C 31 whose power-law slope changes significantly on the scales sampled by our data. The power spectrum can only have the form expected for Kolmogorov turbulence on scales <5 kpc. On larger scales we find a flatter slope. We also compare the global variations of RM across 3C 31 with the results of three-dimensional simulations of the magnetic-field fluctuations in the surrounding magnetoionic medium. We show that our data are consistent with a field distribution that favours the plane perpendicular to the jet axis - probably because the radio source has evacuated a large cavity in the surrounding medium. We also apply our analysis techniques to the case of Hydra A, where the shape and the size of the cavities produced by the source in the surrounding medium are known from X-ray data. (Abridged)
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